Detection and mass spectrometry identification of protein changes in low-abundance tissue using CyDye DIGE Fluor saturation dyes

Traditional two-dimensional electrophoresis (2-DE) is a wellestablished technique for protein analysis, but its use in identifying changes in protein abundance in tissue samples has been limited by system variability and low sensitivity. These limitations have largely precluded its use to identify protein differences in scarce materials such as those recovered from laser-capture microdissection (LCM).